ABSTRACT
Los trastornos temporomandibulares (TTMs) involucran los músculos de la masticación, articulación temporomandibular (ATM) y otras estructuras asociadas a la cavidad oral y representan un conjunto de disfunciones de la articulación, a la cual se le asocia una naturaleza multifactorial, condición que puede llegar a tener de un 50 a 93 por ciento de prevalencia en la población y cuya etiología es asociada a estrés y/o estados psicológicos alterados. Se efectuó un estudio descriptivo observacional de corte transversal con el objetivo de determinar la prevalencia de Trastornos Ansiosos y TTM, en funcionarios del Centro de Salud Familiar (CESFAM) Tucapel, sector Barrio Norte, en la comuna de Concepción. De los 97 funcionarios, 79 aceptaron voluntariamente a participar del estudio y todos trabajan directamente con pacientes en un sector con numerosos problemas socioeconómicos y laborales. Se aplicó una encuesta orientada a la pesquisa tanto de patologías psicológicas, como signos y síntomas de TTM, además de un examen extra e intraoral complementario. En los encuestados se encontró trastornos ansiosos y estados asociados a burnout en un 81 y 27,8 por ciento respectivamente, además había TTM en un 98.7 por ciento de origen multifactorial, pero presente en todos los individuos encuestados y examinados con trastornos ansiosos y estados asociados a burnout.
Temporomandibular disorders (TMDs) involve the muscles of mastication, temporomandibular joint (TMJ) and other structures associated with the oral cavity and represent a multifactorial nature of disorders associated to this joint, condition that is in the range of 50 to 93 percent of prevalence in the population and whose etiology is associated with stress and / or altered psychological states. The present study is a descriptive cross-sectional observational in order to determine the prevalence of anxiety disorders and TMD in workers of the Tucapel Family Health Center (CESFAM), Barrio Norte, in Concepcións commune. Of the 97 staff, 79 agreed to take part voluntarily of the study, all of these people also work directly with patients at a sector with numerous socioeconomic and employment problems. A survey aimed toward the inquiry of psychological, TMD (signs and symptoms) and extra-intra oral complementary examination, was conducted. Anxiety disorders and burnout associated conditions were found (81 and 27.8 percent respectively), multifactorial TMD was also reported (98.7 percent) but present in all individuals with anxiety disorders and conditions associated to burnout.
Subject(s)
Humans , Male , Female , Burnout, Professional/epidemiology , Health Personnel/psychology , Anxiety Disorders/epidemiology , Temporomandibular Joint Disorders/psychology , Burnout, Professional/complications , Chile , Cross-Sectional Studies , Health Centers , Prevalence , Self Concept , Surveys and Questionnaires , Anxiety Disorders/complications , Temporomandibular Joint Disorders/epidemiologyABSTRACT
This study evaluated the attachment, chemo-attractive, proliferative and mineralization inductive potential of a bovine cementum extract (CPE) on newborn murine dental follicle cells (MDFC) in vitro. Cementum extract was partially purified by DEAE-cellulose chromatografy. A band representing and Mr of 55,000 was excised form the fel and the protein (s) were electroeluted. Attachment assays revealed that CPE (1.0 µg/ml) promoted MDFC attachment by 96 percent in comparison with collagen type I (5 µg/ml), and was five-fold greater compared with serum-free media (SFM), (p<0.05). Between 1 and 5 days CPE at 1.0 µg/ml and collagen type I at 5 µg/ml sustained more than 75 percent attachement and spreading of MDFC when compared to SFM (P<0.05). Contrary to other reports, fibronectin (0.5 µg/ml) was more potent than CPE in promoting MDFC chemoattraction (P<0.05). MDFC proliferation was stimulated by CPE (0.125 µg/ml), but this response was elicited only when CPE was used together with 10 percent FBS (37.3 percent) or 0.2 percent FBS (76 percent) (p<0.05). Alkaline phosphatase expression by MDFC was increased by CPE (1.0 µg/ml), in comparison to the control. Calcium deposits were detected by von Kossa staining in 14-day MDFC cultures treated with CPE. Nodule formation and its mineralization in long-term MDFC cultures were induced by CPE (1.0 µg/ml). Molecules(s) contained in CPE appear to regulate various biological activities in MDFC, indicating that CPE could play a key role in selecting progenitor cells required for the process of cementogenesis during development
Subject(s)
Animals , Cell Adhesion Molecules , Chemotaxis/drug effects , Dental Cementum/chemistry , Dental Sac/cytology , Dental Sac/drug effects , Cell Division , In Vitro Techniques , Proteins/pharmacology , Tissue Extracts/pharmacologyABSTRACT
Hertwing's epithelial root sheath (HERS) cells were isolated and recombined with ectomesenchymal cells in vitro utilizing extracellular matrix components as substrate. After 14 days in culture, HERS cells were differnetiated and exhibited a stratified organization. These features resembled those observed in vivo as epithelial rests of Malassez. A mineralization process was also present in HERS cells, in which calcium salts were deposited. This mineralization was correlated with the strong immunoexpression of osteopontin by HERS. The results obtained add support to the possible role of HERS in the secretion of Hypocalcified material on the root during early cementogenesis